DNA-/RNA-Dekontamination. Frisch aus einer Tablette.

Key result: after 30 seconds, no intact pUC18 plasmid band remains in any DN-X® lane – at 50 ng and at 150 ng DNA. In the comparator lanes, distinct bands remain.

Each curve shows the signal along one gel lane, from the well (0) to the end of the gel (1). Intact (native) plasmid DNA runs high in the gel; smaller DNA fragments run further down.

Grey dashed and dotted lines – untreated positive control (C): pUC18 plasmid DNA without any treatment, one control on each gel. Its peaks mark the positions of the intact plasmid bands.

Dark blue line – comparator: a commercially available ready-to-use DNA/RNA decontamination solution. Mean of 3 lanes; the shaded area shows the range.

Magenta line – DN-X®: freshly prepared working solution (1 tablet in 250 ml distilled water). Mean of 3 lanes; the shaded area shows the range.

Reading the profiles: In the DN-X® lanes, the signal at the positions of the intact plasmid bands is at the level of the DNA-free blank lanes. The remaining DN-X® signal lies further down the gel, i.e. smaller DNA fragments. In the comparator lanes, distinct bands remain.

Own densitometric evaluation by Allvater Biosolutions of the original gel images from an external laboratory (agarose gel electrophoresis, test dated 13 October 2026; 50 ng and 150 ng pUC18 plasmid DNA in 10 µl, treated with 5 µl solution for 30 and 60 seconds at room temperature). The gel images were taken with a mobile phone, so the evaluation is semi-quantitative. Each profile is normalised to the untreated control on the same gel; no background correction applied. Single experiment under the conditions described – no guarantee of complete decontamination.